Automated single-slide staining device

A simple apparatus and method is disclosed for making individual single Gram stains on bacteria inoculated slides to assist in classifying bacteria in the laboratory as Gram-positive or Gram-negative. The apparatus involves positioning a single inoculated slide in a stationary position and thereafter automatically and sequentially flooding the slide with increments of a primary stain, a mordant, a decolorizer, a counterstain and a wash solution in a sequential manner without the individual lab technician touching the slide and with minimum danger of contamination thereof from other slides

Microbiological surface sampling cart

Mobile sampling cart automatically swabs surfaces for the recovery of microorganisms. Unit operates without human involvement and provides for control of swabbing speed, rotation of cotton swab, and the pressure and angle applied to swab. Capability of reverse direction is also available. Sampling cart use is limited to flat surfaces

Apparatus for microbiological sampling

An automatic apparatus is described for microbiologically sampling surface using a cotton swab which eliminates human error. The apparatus includes a self-powered transport device, such as a motor-driven wheeled cart, which mounts a swabbing motor drive for a crank arm which supports a swab in the free end thereof. The swabbing motor is pivotably mounted and an actuator rod movable responsive to the cart traveling a predetermined distance provides lifting of the swab from the surface being sampled and reversal of the direction of travel of the cart

Automatic microbial transfer

Device can transfer metabolites or inhibitory agents to broth cultures of bacteria, in various stages of growth, for study. It also has application in transfer of other micro-organisms, such as yeasts, and could be useful in clinical and research laboratories. Device has been used for wide variety of purposes in experimental situations

Automated single-slide staining system

Apparatus developed to Gram-stain single slides automatically is flexible enough to accommodate other types of staining procedures. Method frees operator and eliminates necessity for subjective evaluations as to length of staining or decolorizing time

Automatic agar tray inoculation device

Automatic agar tray inoculation device is simple in design and foolproof in operation. It employs either conventional inoculating loop or cotton swab for uniform inoculation of agar media, and it allows technician to carry on with other activities while tray is being inoculated

Measuring micro-organism gas production

Transducer, which senses pressure buildup, is easy to assemble and use, and rate of gas produced can be measured automatically and accurately. Method can be used in research, in clinical laboratories, and for environmental pollution studies because of its ability to detect and quantify rapidly the number of gas-producing microorganisms in water, beverages, and clinical samples

Visualisation of the complexome.

Proteins and other molecules interact with each other to form macromolecular complexes, which are the functional units inside the cell. Together, these make up the complexome. To date, there are 4 large-scale datasets for protein complexes in Saccharomyces cerevisiae. These were established by affinity purification and mass spectrometry. Of these, one featured protein sharing between complexes (Gavin et al.1). In this study, they proposed a ʻcore-module-attachmentʼ model. Core proteins are always present within a complex. Attachment proteins are single proteins that are sometimes but not always in a complex. Modules are groups of two or more strongly associated attachment proteins present in more than one complex. The aim of this study is to visualise the complexome as a network. This should provide a unique ʻhigh-levelʼ and biologically relevant view of the interactome

Management of the Rice Tungro Virus Vector \u3ci\u3eNephotettix virescens\u3c/i\u3e (Homoptera: Cicadellidae) with Controlled-Release Formulations of Carbofuran

Field trials were conducted in lowland flooded rice in the Philippines to evaluate a number of carbofuran controlled-release formulations in comparison with commercial formulations. The test formulations were based on a biodegradable matrix of pine kraft lignin and were used as granules of different sizes and also in the form of small strips. The release rates were assessed under field conditions by bioassaying rice plants in the field, using adult rice green leafhopper, Nephotettix virescens Distant. The lignin formulations with a high level of active ingredient (15–45% by weight) gave as good or better control than the commercial 3% granules in tests based on three application techniques: broadcast into the floodwater, soil incorporation, and root zone injection. The improvements in control levels of green leafhoppers were most marked with soil incorporation and root zone application. The best lignin-based formulation reduced levels of tungro virus infection from 23% for a conventional flowable carbofuran formulation to 1.0% at an application rate of 0.5 kg (AI)/ha. At the same rate, the grain yield was increased from 3.56 t/ha to 5.5 t/ha, using the controlled-released formulation